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임상발표자료

2004 ICS  Paris

158

Lee E¹, Byun S², Kim J H³, Yeo W¹

1. Seoul National University Hospital, 2. Seoul National University Bundang Hospital,

3. Kangwon National University Hospital

 

IMAPACT OF MAGNETIC FIELDS ON THE GROWTH OF CANCER CELL LINES OF GENITOURINARY SYSTEM

 

Hypothesis / aims of study

 Perineal magnetic stimulation for urinary incontinence was first described in 1999 by Galloway et al and it showed encouraged results [1]. The magnetic field penetrates in all body tissues without significant alteration, falling off only as the inverse square of the distance and passing uninterrupted through clothing. As a consequence, it is not necessary to have the patient undress for treatment.

 Recent epidemiological studies on occupational and residential exposure to electric magnetic fields are concerned with the biological effects of 50-60Hz fields, particularly with determining an increase in cancer incidence in individuals exposed to these types of radiation [2.3]. Thus magnetic field exposure may be associated with development and progression of tumor.

 The aim of this study was to investigate the interaction between commonly used magnetic field (MF) exposure for urinary incontinence and growth of genitourinary cancer cell lines.

 

Study design, materials and methods

Two bladder and two prostate cancer cell lines were used: T24, 253J, DU-145 and LNCAP.

The cells were grown in RPMI 1640 culture medium with 10% FBS and 1% penicillin/streptomycin at 37±0.5 and 5% CO. The cells were seeded in a concentration of 1 X 10cells/ml in a 96-well plate. Each cell line was categorized into 3 groups, including control (no exposure), 10Hz group (9mT in magnetic field flux density) and 50Hz group (12mT in magnetic field flux density). The control comprised of 16 wells and the other groups 8 wells.

 Magnetic exposure comprised of 5 sessions of 30 minutes each. It was done daily using a specially designed chair (Mcube Technology, Inc., Seoul, Korea). The session included 30 minutes of intermittent low frequency stimulation (3 seconds at 10Hz and 6 seconds off) for the 10Hz group and 30 minutes of intermittent high frequency stimulation (3 seconds at 50Hz and 6 seconds off) for the 50Hz group. After 5 days of exposure, the cell growth was evaluated using MTT assay. Statistical analysis was done using Mann-Whitney U test.

 

Results

Magnetic field exposure reduced cell growth in the 10Hz and 50Hz groups compared to the control group (Table 1 and 2) and it was statistically significant (P<0.05).

Table 1. Tumor growth kinetics (in Optical Density ± S.D) of the 10Hz group

 

Control

10Hz group

P value

T24

3.26 ± 0.2

2.82 ± 0.11

0.000

253J

3.64 ± 0.18

3.32 ± 0.48

0.006

DU-145

3.63 ± 0.11

3.41 ± 0.09

0.001

LNCAP

3.43 ± 0.17

3.27 ± 0.16

0.045

 

Table 2. Tumor growth kinetics (in Optical Density ± S.D) of the 50Hz group

 

Control

50Hz group

P value

T24

3.26 ± 0.2

2.69 ± 0.09

0.000

253J

3.64 ± 0.18

3.33 ± 0.17

0.001

DU-145

3.63 ± 0.11

3.13 ± 0.12

0.000

LNCAP

3.43 ± 0.17

3.11 ± 0.12

0.006

 

Interpretation of results

Magnetic filed exposure with usual dosage for urinary incontinence did not increase the tumor growth.

This might suggest that potential tumor growth could not be expected in the patients with hidden and established genitourinary malignancies for treatment of urinary incontinence.